PCR: | Psen1 | ||||
Name of Positive CTRL: | Psen1 - mutant | Annealing Temperature: | 69.0 °C | ||
Number of cycles: | 35.0 | Extension Time: | 60 s | ||
Hot Start: | HS 70 | ||||
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
Psen1 fwd | 925 | 100 µM | 10.0 µl for 1ml | AGGCAGGAAGATCACGTGTTCAAGTAC | |
Psen1 rev | 926 | 100 µM | 10.0 µl for 1ml | CACACGCACACTCTGACATGCACAGGC | |
Expected Bands: | no expected bands in db | ||||
More Information in PDF: | view PDF | ||||