PCR: | Pdgfra flox wt |
Name of Positive CTRL: | Pdgfra flox wt |
Annealing Temperature: | 60.0 °C | Number of cycles: | 0.0 |
Extension Time: | 0 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mTUBB5-247 F | 277 | 100 µM | 5.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 5.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
| oIMR7801 | 805 | 100 µM | 20.0 µl for 1ml | CCCTTGTGGTCATGCCAAAC |
| oIMR7802 | 806 | 100 µM | 20.0 µl for 1ml | GCTTTTGCCTCCATTACACTGG |
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Expected Bands: | Band Size: 247.0 | Description: 247 mTUBB | (text displayed in the image) |
| Band Size: 451.0 | Description: 451 wt | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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