PCR:	TITL-GCaMP6f wt
Name of Positive CTRL:	TITL-GCaMP6f wt
Annealing Temperature:	58.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	TIGRE-wt-for	787	100 µM	20.0 µl for 1ml	GTCAGGCACAAGAGGTCTACATTC
	TIGRE-wt-rev	788	100 µM	20.0 µl for 1ml	GGTGGCACACACCTTTAATCCC
Expected Bands:	Band Size: 380.0	Description: 380 wt	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
More Information in PDF:		view PDF