Name of Positive CTRL:TITL-GCaMP6f mut
Annealing Temperature:58.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
GCaMP6f-com-for785100 µM20.0 µl for 1mlATAGATCCACCTGCCTCTGC
GCaMP6f-mut-rev786100 µM20.0 µl for 1mlTCCCCTGGCACAACGTAAG
Expected Bands:Band Size: 275.0Description: 275 mut(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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