Name of Positive CTRL:FSN
Annealing Temperature:60.0 °C
Number of cycles:40.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
P1-TTC7 FW771100 µM10.0 µl for 1mlCAAGATGCTTGCTTCTGG
P2-ETn RV772100 µM17.5 µl for 1mlCCGATACGAAGATCCTTTCC
wt_TTC7 RV770100 µM5.0 µl for 1mlGAAGAGCAGCGCTAGCAAGT
Expected Bands:Band Size: 191.0Description: 191 wt(text displayed in the image)
Band Size: 450.0Description: 450 mutant(text displayed in the image)
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