PCR: | IRES-DTR |
Name of Positive CTRL: | IRES-DTR |
Annealing Temperature: | 62.0 °C | Number of cycles: | 35.0 |
Extension Time: | 10 s | Hot Start: | HS 62 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| 5248 | 769 | 100 µM | 10.0 µl for 1ml | GGAAAATGCATGGTCTATCCTAGC |
| 5424 | 768 | 100 µM | 20.0 µl for 1ml | GGGAGAGGGGCATAACTTCGTATAGC |
| 5454 | 767 | 100 µM | 30.0 µl for 1ml | GACTCAGTGGCTATTCCGTCTACTTG |
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Expected Bands: | Band Size: 452.0 | Description: 452 tg | (text displayed in the image) |
| Band Size: 562.0 | Description: 562 wt | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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