PCR:	LAG3 KO
Name of Positive CTRL:	LAG3 KO
Annealing Temperature:	60.0 °C
Number of cycles:	30.0
Extension Time:	150 s
Hot Start:	HS 53
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	G25a	754	100 µM	20.0 µl for 1ml	AAATCACAACCCCTAGCCATTGCTC
	G25c	756	100 µM	20.0 µl for 1ml	CGATACCGTAAAGCACGATTAAGC
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: 247 mTUBB	(text displayed in the image)
	Band Size: 1121.0	Description: 1121 ko	(text displayed in the image)
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