PCR: Vglut2-ires-cre
Name of Positive CTRL:Vglut2-ires-cre
Annealing Temperature:65.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
11642742100 µM10.0 µl for 1mlATCGACCGGTAATGCAGGCAA
13126743100 µM20.0 µl for 1mlCGGTACCACCAAATCTTACGG
13127744100 µM10.0 µl for 1mlCATGGTCTGTTTTGAATTCAG
Expected Bands:Band Size: 299.0Description: 299 wt(text displayed in the image)
Band Size: 850.0Description: 850 mut(text displayed in the image)
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