PCR: | PKBa |
Name of Positive CTRL: | PKBa |
Annealing Temperature: | 62.5 °C | Number of cycles: | 20.0 |
Extension Time: | 120 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| PKBalpha3 | 740 | 100 µM | 10.0 µl for 1ml | TGAAGCAGGCCTAGAGCCCCATG |
| PKBalpha5 | 739 | 100 µM | 20.0 µl for 1ml | AGACTCTGAGCATCATCCCTGGG |
| PKBalphaLacZ-2 | 741 | 100 µM | 10.0 µl for 1ml | CGTCTGGCCTTCCTGTAGCCAG |
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Expected Bands: | Band Size: 200.0 | Description: 200 ko | (text displayed in the image) |
| Band Size: 295.0 | Description: 295 wt | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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