PCR: Sall1 flox
Name of Positive CTRL:Sall1 flox
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Sall1 flox2731100 µM10.0 µl for 1mlCCTCTGCCCGAGAGATCG
Sall1 flox3732100 µM10.0 µl for 1mlGGCGCGTCTGATTTTATTTC
Sall1 flox5733100 µM10.0 µl for 1mlAGGAACACTCACGAAATGGG
Expected Bands:Band Size: 220.0Description: 220 wt(text displayed in the image)
Band Size: 280.0Description: 280 mut(text displayed in the image)
Band Size: 350.0Description: 350 delta(text displayed in the image)
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