PCR: | mCrb1 |
Name of Positive CTRL: | mCrb1 |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mCrb1 mF1 | 722 | 100 µM | 10.0 µl for 1ml | GTGAAGACAGCTACAGTTCTGATC |
| mCrb1 mF2 | 723 | 100 µM | 10.0 µl for 1ml | GCCCCTGTTTGCATGGAGGAAACTTGGAAGACAGCTACAGTTCTTCTG |
| mCrb1 mR | 724 | 100 µM | 20.0 µl for 1ml | GCCCCATTTGCACACTGATGAC |
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Expected Bands: | Band Size: 220.0 | Description: 220mCrb1wt | (text displayed in the image) |
| Band Size: 244.0 | Description: 244mCrb1Rd | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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