PCR: Gkn1wt
Name of Positive CTRL:Gkn1wt
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:40 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Reg-Gkn1-wtF710100 µM20.0 µl for 1mlGTCTAGAGGAGTGATTCTCGACCTTCC
Reg-Gkn1-wtR709100 µM20.0 µl for 1mlGCATGATGTTATCTCCTGTAAATGAGG
Expected Bands:Band Size: 284.0Description: 284 wt(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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