PCR: | Gkn1 PreCre |
Name of Positive CTRL: | Gkn1 PreCre |
Annealing Temperature: | 55.0 °C | Number of cycles: | 35.0 |
Extension Time: | 40 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| mTUBB5-247 F | 277 | 100 µM | 10.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 10.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
| Reg-Gkn1-R | 708 | 100 µM | 20.0 µl for 1ml | GTCTCTCATTCCCAGTGTCTCCTCC |
| Reg-NeoF | 707 | 100 µM | 20.0 µl for 1ml | GCAGCCTCTGTTCCACATACACTTCA |
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Expected Bands: | Band Size: 247.0 | Description: mTUBB-247 | (text displayed in the image) |
| Band Size: 515.0 | Description: 515 PreCre | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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