PCR: Rosa-Cas9 wt
Name of Positive CTRL:Rosa-Cas9 wt
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Rosa-Cas9 F343100 µM20.0 µl for 1mlaag gga gct gca gtg gag ta
Rosa-Cas9 wt699100 µM20.0 µl for 1mlCCGAAAATCTGTGGGAAGTC
Expected Bands:Band Size: 297.0Description: 297 wt(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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