PCR:	Akt1
Name of Positive CTRL:	Akt1
Annealing Temperature:	63.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Akt1-6F	691	100 µM	20.0 µl for 1ml	CTGGACCTGTCTCCTACCC
	Akt1-6R	692	100 µM	20.0 µl for 1ml	CAACCCTGTGGCTCTGTG
Expected Bands:	Band Size: 310.0	Description: 310 wt	(text displayed in the image)
	Band Size: 454.0	Description: 454 LoxP	(text displayed in the image)
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