PCR: Akt1
Name of Positive CTRL:Akt1
Annealing Temperature:63.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Akt1-6F691100 µM20.0 µl for 1mlCTGGACCTGTCTCCTACCC
Akt1-6R692100 µM20.0 µl for 1mlCAACCCTGTGGCTCTGTG
Expected Bands:Band Size: 310.0Description: 310 wt(text displayed in the image)
Band Size: 454.0Description: 454 LoxP(text displayed in the image)
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