PCR: Kras wt/mut
Name of Positive CTRL:Kras mut/wt
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
22907 Kras wt F695100 µM10.0 µl for 1mlTGTCTTTCCCCAGCACAGT
22908 Kras R696100 µM20.0 µl for 1mlCTGCATAGTACGCTATACCCTGT
oIMR9592 Kras mut F697100 µM10.0 µl for 1mlGCAGGTCGAGGGACCTAATA
Expected Bands:Band Size: 100.0Description: 100 mut(text displayed in the image)
Band Size: 250.0Description: 250 wt(text displayed in the image)
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