PCR: Cacna2d1 wt
Name of Positive CTRL:Cacna2d1 wt
Annealing Temperature:52.0 °C
Number of cycles:25.0
Extension Time:60 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Primer A - α2δ-1680100 µM20.0 µl for 1mlGAGCTTTCTTTCTTCTGATTCCAC
Primer C - α2δ-1682100 µM20.0 µl for 1mlACATTCTCAAGACTGTAGGCAGAG
Expected Bands:Band Size: 346.0Description: 346 wt(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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