Name of Positive CTRL:ROSA26-mT/mG
Annealing Temperature:61.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
IMR7318667100 µM20.0 µl for 1mlCTCTGCTGCCTCCTGGCTTCT
IMR7319668100 µM10.0 µl for 1mlCGAGGCGGATCACAAGCAATA
IMR7320669100 µM10.0 µl for 1mlTCAATGGGCGGGGGTCGTT
Expected Bands:Band Size: 250.0Description: 250 mut(text displayed in the image)
Band Size: 330.0Description: 330 wt(text displayed in the image)
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