PCR: RANKflox
Name of Positive CTRL:RANKflox
Annealing Temperature:64.0 °C
Number of cycles:35.0
Extension Time:40 s
Hot Start:HS 65
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
P105659100 µM10.0 µl for 1mlCTGGTGGTTGTTCTCCTGGTGTCAT
P87657100 µM20.0 µl for 1mlGGCAGAACTCGGATGCACAGATTGG
P88658100 µM30.0 µl for 1mlAGTGTGCCTGGCATGTGCAGACCTT
Expected Bands:Band Size: 256.0Description: 256 wt(text displayed in the image)
Band Size: 390.0Description: 390 floxed(text displayed in the image)
Band Size: 566.0Description: 566 delta(text displayed in the image)
 
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