PCR: | ZH3 RFLP |
Name of Positive CTRL: | ZH3 RFLP +/- |
Annealing Temperature: | 58.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | HS 57 + Dig |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| AL95 | 655 | 100 µM | 10.0 µl for 1ml | AGGGTTGACGCCATGACTTT |
| AL96 | 656 | 100 µM | 10.0 µl for 1ml | TATGGGTACCCCCTCCTTGG |
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Expected Bands: | Band Size: 146.0 | Description: 146 wt | (text displayed in the image) |
| Band Size: 250.0 | Description: 250 wt | (text displayed in the image) |
| Band Size: 388.0 | Description: 388 ZH3 | (text displayed in the image) |
| Band Size: 460.0 | Description: het unsp | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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