PCR: 129mmHuTg
Name of Positive CTRL:129mmHuTg
Annealing Temperature:53.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
129m hutg-fwd653100 µM20.0 µl for 1mlCAGTCATTATGGCGAACCTT
129m hutg-rev654100 µM20.0 µl for 1mlGACCTTCCTCATCCCACT
Actin fwd114100 µM5.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM5.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Expected Bands:Band Size: 500.0Description: 500 actin(text displayed in the image)
Band Size: 750.0Description: 750 tg(text displayed in the image)
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