PCR: mGluR5
Name of Positive CTRL:mGluR5 wt/ko
Annealing Temperature:58.0 °C
Number of cycles:35.0
Extension Time:40 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
grmko-common645100 µM20.0 µl for 1mlCACATGCCAGGTGACATCAT
grmko-mut_RV647100 µM10.0 µl for 1mlCACGAGACTAGTGAGACGTG
grmko-wt_RV646100 µM10.0 µl for 1mlCCATGCTGGTTGCAGAGTAA
Expected Bands:Band Size: 442.0Description: 442 wt(text displayed in the image)
Band Size: 650.0Description: 650 mut(text displayed in the image)
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