Name of Positive CTRL:DATcre
Annealing Temperature:58.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
DATcre_rev652100 µM20.0 µl for 1mlCGGCAATATGGTGGAAAATAAC
DATcre_wt_fwd651100 µM30.0 µl for 1mlTGGTGTAAAGTGGAAGGAGAC
DATcre_wt_rev650100 µM10.0 µl for 1mlTTTCTTGACGTGTTGGTTTCC
Expected Bands:Band Size: 97.0Description: 97 wt(text displayed in the image)
Band Size: 141.0Description: 141 tg(text displayed in the image)
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