PCR:	JAK2-V617F_KI
Name of Positive CTRL:	JAK2-V617F_KI
Annealing Temperature:	59.0 °C
Number of cycles:	35.0
Extension Time:	30 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	JAK2-V617F_KI F	637	100 µM	20.0 µl for 1ml	GTACGCTGTCCACCCAGATT
	JAK2-V617F_KI R_KI	670	100 µM	10.0 µl for 1ml	TTCACATCTTGTTCCTGTTGC
	JAK2-V617F_KI R_wt	638	100 µM	10.0 µl for 1ml	TGCCTCCATTTCTGTCATTG
Expected Bands:	no expected bands in db
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