Name of Positive CTRL:FF1
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
FF1 F633100 µM20.0 µl for 1mlGAGCAAGCTTTCTCACAAGC
FF1 R634100 µM20.0 µl for 1mlAATTCTGCCCACTTTGGTGC
mTUBB5-247 F277100 µM10.0 µl for 1mlCGG CCA CCA TGA GCG GCG TC
mTUBB5-247 R392100 µM10.0 µl for 1mlGTG AGG TAC CGG CCG TGG CG
Expected Bands:Band Size: 247.0Description: mTUBB5-247(text displayed in the image)
Band Size: 500.0Description: 500 FF1(text displayed in the image)
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