PCR:	villin-Cre
Name of Positive CTRL:	villin-Cre
Annealing Temperature:	62.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	VilCre1	631	100 µM	10.0 µl for 1ml	gtg tgg gac aga gaa caa acc
	VilCre2	632	100 µM	10.0 µl for 1ml	aca tct tca ggt tct gcg gg
Expected Bands:	Band Size: 500.0	Description: 500 actin	(text displayed in the image)
	Band Size: 1100.0	Description: 1100 tg	(text displayed in the image)
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