PCR: | Prnp-GFP |
Name of Positive CTRL: | Prnp-GFP |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 30 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| 75 wt | 619 | 100 µM | 10.0 µl for 1ml | AGATCCGCCACAACATCGAGG |
| 77 common | 621 | 100 µM | 20.0 µl for 1ml | GAGCTACAGGTGGATAACCCC |
| 79 ko | 620 | 100 µM | 10.0 µl for 1ml | GAGCAGATGTGCGTCACCCAG |
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Expected Bands: | Band Size: 204.0 | Description: 204 wt | (text displayed in the image) |
| Band Size: 285.0 | Description: 285 GFP | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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