Name of Positive CTRL:Prnp-GFP
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
75 wt619100 µM10.0 µl for 1mlAGATCCGCCACAACATCGAGG
77 common621100 µM20.0 µl for 1mlGAGCTACAGGTGGATAACCCC
79 ko620100 µM10.0 µl for 1mlGAGCAGATGTGCGTCACCCAG
Expected Bands:Band Size: 204.0Description: 204 wt(text displayed in the image)
Band Size: 285.0Description: 285 GFP(text displayed in the image)
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