PCR:	FoxP3-Mal wt
Name of Positive CTRL:	FoxP3-Mal wt
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	FoxP3-001	612	100 µM	10.0 µl for 1ml	GCC AAT CCC AGC CTA GCC CCT AGT
	FoxP3-002	613	100 µM	10.0 µl for 1ml	CAG CTG ACC ACA GTT TAT TGG ATG
Expected Bands:	Band Size: 500.0	Description: 500 actin	(text displayed in the image)
	Band Size: 650.0	Description: 650 FoxP3	(text displayed in the image)
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