Name of Positive CTRL:TetO
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
TetO 1-17615100 µM10.0 µl for 1mlGCGGCCGCCAACTCTCG
TetO 419-395616100 µM10.0 µl for 1mlTCAAAACAGCGTGGATGGCGTCTC
Expected Bands:Band Size: 420.0Description: 420 TetO(text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
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