Name of Positive CTRL:LIF-EGFP
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:35 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
LIF-EGFP fwd611100 µM20.0 µl for 1mlcgccgccgggatcactctcg
LIF-EGFP rev610100 µM20.0 µl for 1mlgtcccaaaccccagcacatt
Expected Bands:Band Size: 135.0Description: 135 tg(text displayed in the image)
Band Size: 500.0Description: 500actin(text displayed in the image)
More Information in PDF: view PDF