PCR: Ifnar1
Name of Positive CTRL:Ifnar1
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
9850 mut601100 µM10.0 µl for 1mlCGA GGC GAA GTG GTT AAA AG
9851 wt602100 µM10.0 µl for 1mlACG GAT CAA CCT CAT TCC AC
IMR8963 common603100 µM20.0 µl for 1mlAAT TCG CCA ATG ACA AGA CG
Expected Bands:Band Size: 155.0Description: 155 wt(text displayed in the image)
Band Size: 250.0Description: 250 mutant(text displayed in the image)
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