PCR: | Ifnar1 |
Name of Positive CTRL: | Ifnar1 |
Annealing Temperature: | 55.0 °C | Number of cycles: | 35.0 |
Extension Time: | 30 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| 9850 mut | 601 | 100 µM | 10.0 µl for 1ml | CGA GGC GAA GTG GTT AAA AG |
| 9851 wt | 602 | 100 µM | 10.0 µl for 1ml | ACG GAT CAA CCT CAT TCC AC |
| IMR8963 common | 603 | 100 µM | 20.0 µl for 1ml | AAT TCG CCA ATG ACA AGA CG |
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Expected Bands: | Band Size: 155.0 | Description: 155 wt | (text displayed in the image) |
| Band Size: 250.0 | Description: 250 mutant | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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