PCR: Vil-Cre
Name of Positive CTRL:Vil-Cre
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:30 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
2kbseqS589100 µM20.0 µl for 1mlCAA GCC TGG CTC GAC GGC C
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
Cre198as590100 µM20.0 µl for 1mlCGC GAA CAT CTT CAG GTT CT
Expected Bands:Band Size: 300.0Description: 300 VilCre(text displayed in the image)
Band Size: 500.0Description: 500 actin(text displayed in the image)
 
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