PCR: | PTEN | ||||
Name of Positive CTRL: | PTEN | Annealing Temperature: | 50.0 °C | ||
Number of cycles: | 35.0 | Extension Time: | 30 s | ||
Hot Start: | HS 57 | ||||
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
F-PTEN | 582 | 100 µM | 10.0 µl for 1ml | CTC CTC TAC TCC ATT CTT CCC | |
R-PTEN | 583 | 100 µM | 10.0 µl for 1ml | ACT CCC ACC AAT GAA CAA AC | |
Expected Bands: | Band Size: 228.0 | Description: 228 WT | (text displayed in the image) | ||
Band Size: 335.0 | Description: 335 flox | (text displayed in the image) | |||
More Information in PDF: | view PDF | ||||