PCR: | Msr1 |
Name of Positive CTRL: | Msr1 |
Annealing Temperature: | 57.0 °C | Number of cycles: | 35.0 |
Extension Time: | 30 s | Hot Start: | HS 57 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| IMR5284 mut | 579 | 100 µM | 10.0 µl for 1ml | TCA AAC TCA AAA GCC GAC CTT AT |
| IMR5285 common | 580 | 100 µM | 20.0 µl for 1ml | AGG ACT TGG AGA TTG CAT CCA |
| IMR5286 wt | 581 | 100 µM | 10.0 µl for 1ml | GTG GTT TCC AAA TGT GTC AGT TTC |
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Expected Bands: | Band Size: 154.0 | Description: 154 wt | (text displayed in the image) |
| Band Size: 202.0 | Description: 202 mut | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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