PCR: | MyD88 |
Name of Positive CTRL: | MyD88 |
Annealing Temperature: | 60.0 °C | Number of cycles: | 35.0 |
Extension Time: | 60 s | Hot Start: | HS 57 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| MyD88 extra | 576 | 100 µM | 10.0 µl for 1ml | ATCGGCTTAAGTTGTGTGTGTCCGACC |
| MyD88 wild | 577 | 100 µM | 10.0 µl for 1ml | AAGGCCAAAGGGTGTGGTATTAGGACC |
| Neo1500 | 578 | 100 µM | 10.0 µl for 1ml | ATCGCCTTCTATCGCCTTCTTGACGAG |
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Expected Bands: | Band Size: 300.0 | Description: 300 ko | (text displayed in the image) |
| Band Size: 500.0 | Description: 500 wt | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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