PCR:	PrP-Ngsk neo
Name of Positive CTRL:	PrP-Ngsk neo
Annealing Temperature:	55.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	Ngsk neo 1 (657)	564	100 µM	20.0 µl for 1ml	GGT GCC CTG AAT GAA CTG CA
	Ngsk neo 2 (658)	565	100 µM	20.0 µl for 1ml	GGT AGC CGG ATC AAG CGT AT
Expected Bands:	Band Size: 227.0	Description: 227 neo	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
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