PCR:	PrP-Ngsk wt
Name of Positive CTRL:	PrP-Ngsk wt
Annealing Temperature:	55.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	m28s rDNA 225 F	390	10 µM	10.0 µl for 1ml	CAG ACG TGG CGA CCC GCT GA
	m28s rDNA-225 R	276	10 µM	10.0 µl for 1ml	GCC TCA CAC CGT CCA CGG GC
	Ngsk wt 1 (655)	562	100 µM	20.0 µl for 1ml	CCG CTA CCC TAA CCA AGT GT
	Ngsk wt 2 (656)	563	100 µM	20.0 µl for 1ml	CCT AGA CCA CGA GAA TGC GA
Expected Bands:	Band Size: 225.0	Description: m28	(text displayed in the image)
	Band Size: 364.0	Description: wt	(text displayed in the image)
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