PCR: PrP-Ngsk wt
Name of Positive CTRL:PrP-Ngsk wt
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
m28s rDNA 225 F39010 µM10.0 µl for 1mlCAG ACG TGG CGA CCC GCT GA
m28s rDNA-225 R27610 µM10.0 µl for 1mlGCC TCA CAC CGT CCA CGG GC
Ngsk wt 1 (655)562100 µM20.0 µl for 1mlCCG CTA CCC TAA CCA AGT GT
Ngsk wt 2 (656)563100 µM20.0 µl for 1mlCCT AGA CCA CGA GAA TGC GA
Expected Bands:Band Size: 225.0Description: m28(text displayed in the image)
Band Size: 364.0Description: wt(text displayed in the image)
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