PCR:	PDGF-GFP
Name of Positive CTRL:	PDGF-GFP
Annealing Temperature:	64.0 °C
Number of cycles:	6.0
Extension Time:	45 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Actin fwd	114	100 µM	10.0 µl for 1ml	TGT TAC CAA CTG GGA CGA CA
	Actin rev	115	100 µM	10.0 µl for 1ml	GAC ATG CAA GGA GTG CAA GA
	GSeGFP R3	551	100 µM	20.0 µl for 1ml	GGT CGG GGT AGC GGC TGA
	Pdgfrb(31796)F	550	100 µM	20.0 µl for 1ml	GTG GAA GCA GAG AGG AGA GCA TTT G
Expected Bands:	Band Size: 425.0	Description: 425 PDGF	(text displayed in the image)
	Band Size: 500.0	Description: 500 Actin	(text displayed in the image)
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