Name of Positive CTRL:PDGF-GFP
Annealing Temperature:64.0 °C
Number of cycles:6.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
GSeGFP R3551100 µM20.0 µl for 1mlGGT CGG GGT AGC GGC TGA
Pdgfrb(31796)F550100 µM20.0 µl for 1mlGTG GAA GCA GAG AGG AGA GCA TTT G
Expected Bands:Band Size: 425.0Description: 425 PDGF(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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