Name of Positive CTRL:EGFP
Annealing Temperature:60.0 °C
Number of cycles:30.0
Extension Time:45 s
Hot Start:HS 60
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Actin fwd114100 µM10.0 µl for 1mlTGT TAC CAA CTG GGA CGA CA
Actin rev115100 µM10.0 µl for 1mlGAC ATG CAA GGA GTG CAA GA
EGFP_forw548100 µM20.0 µl for 1mlCCT ACG GCG TGC AGt GCT TCA GC
EGFP_rev549100 µM20.0 µl for 1mlCGG CGA GCT GCA CGC TGC GTC CTC
Expected Bands:Band Size: 300.0Description: 300 EGFP(text displayed in the image)
Band Size: 500.0Description: 500 Actin(text displayed in the image)
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