PCR: | EGFP |
Name of Positive CTRL: | EGFP |
Annealing Temperature: | 60.0 °C | Number of cycles: | 30.0 |
Extension Time: | 45 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Actin fwd | 114 | 100 µM | 10.0 µl for 1ml | TGT TAC CAA CTG GGA CGA CA |
| Actin rev | 115 | 100 µM | 10.0 µl for 1ml | GAC ATG CAA GGA GTG CAA GA |
| EGFP_forw | 548 | 100 µM | 20.0 µl for 1ml | CCT ACG GCG TGC AGt GCT TCA GC |
| EGFP_rev | 549 | 100 µM | 20.0 µl for 1ml | CGG CGA GCT GCA CGC TGC GTC CTC |
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Expected Bands: | Band Size: 300.0 | Description: 300 EGFP | (text displayed in the image) |
| Band Size: 500.0 | Description: 500 Actin | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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