PCR:	GCaMP3 wt
Name of Positive CTRL:	GCaMP3 wt
Annealing Temperature:	50.0 °C
Number of cycles:	35.0
Extension Time:	40 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	GCaMP3 F10	523	100 µM	20.0 µl for 1ml	CCA AAG TCG CTC TGA GTT GTT ATC
	GCaMP3 R10	524	100 µM	20.0 µl for 1ml	GAG CGG GAG AAA TGG ATA TG
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: mTUBB 247	(text displayed in the image)
	Band Size: 604.0	Description: 604 wt	(text displayed in the image)
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