PCR:	GCaMP3 mut
Name of Positive CTRL:	GCaMP3 mut
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	40 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	GCaMP3 F9	520	100 µM	20.0 µl for 1ml	CTT CAA GAT CCG CCA CAA CAT CG
	GCaMP3 R9	522	100 µM	20.0 µl for 1ml	TTG AAG AAG ATG GTG CGC TCC TG
	mTUBB5-247 F	277	100 µM	20.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	20.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: mTUBB 247	(text displayed in the image)
	Band Size: 546.0	Description: 506 mut	(text displayed in the image)
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