PCR:	Del-1 wt
Name of Positive CTRL:	Del-1 wt
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	45 s
Hot Start:	HS 57
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	Del-1 fwd	104	100 µM	20.0 µl for 1ml	tcttgaatctctgacagggc
	Del-1 rev	105	100 µM	20.0 µl for 1ml	ctttgccgaactggggcac
	mTUBB short F	527	10 µM	10.0 µl for 1ml	ACCGGGCCCTCACTGTGCCTG
	mTUBB short R	528	10 µM	10.0 µl for 1ml	CGTCCACGGAAGACGGCGGCA
Expected Bands:	Band Size: 118.0	Description: mTUBB5-118	(text displayed in the image)
	Band Size: 180.0	Description: 180 wt	(text displayed in the image)
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