PCR: Del-1 wt
Name of Positive CTRL:Del-1 wt
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:45 s
Hot Start:HS 57
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
Del-1 fwd104100 µM20.0 µl for 1mltcttgaatctctgacagggc
Del-1 rev105100 µM20.0 µl for 1mlctttgccgaactggggcac
mTUBB short F52710 µM10.0 µl for 1mlACCGGGCCCTCACTGTGCCTG
mTUBB short R52810 µM10.0 µl for 1mlCGTCCACGGAAGACGGCGGCA
Expected Bands:Band Size: 118.0Description: mTUBB5-118(text displayed in the image)
Band Size: 180.0Description: 180 wt(text displayed in the image)
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