PCR:	CAR wt
Name of Positive CTRL:	CAR wt
Annealing Temperature:	60.0 °C
Number of cycles:	35.0
Extension Time:	60 s
Hot Start:	HS 60
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	car_1062	477	100 µM	10.0 µl for 1ml	GCA AAC GGA CAG ATG GGA C
	car_1063	478	100 µM	10.0 µl for 1ml	CTC AAC TCC TCC CAC ATT CAG
	mTUBB5-247 F	277	100 µM	10.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	10.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: mTUBB 247	(text displayed in the image)
	Band Size: 663.0	Description: 663 Car wt	(text displayed in the image)
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