Name of Positive CTRL:CR2
Annealing Temperature:55.0 °C
Number of cycles:35.0
Extension Time:60 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
CR1/2 a504100 µM20.0 µl for 1mlTGTCAGGCTCCTCCTAAAATTAT
CR1/2 b505100 µM10.0 µl for 1mlCTTTACAAAGACGGATTTCTATA
CR1/2 c506100 µM10.0 µl for 1mlCCACTGTCCTTTCCTAATAAAATG
Expected Bands:Band Size: 474.0Description: neo(text displayed in the image)
Band Size: 693.0Description: wt(text displayed in the image)
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