PCR:	Kras mut
Name of Positive CTRL:	Kras mut
Annealing Temperature:	65.0 °C
Number of cycles:	35.0
Extension Time:	45 s
Hot Start:	HS 65
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	IMR8273	497	100 µM	20.0 µl for 1ml	CGC AGA CTG TAG AGC AGC G
	IMR8274	498	100 µM	20.0 µl for 1ml	CCA TGG CTT GAG TAA GTC TGC
	mTUBB5-247 F	277	100 µM	8.0 µl for 1ml	CGG CCA CCA TGA GCG GCG TC
	mTUBB5-247 R	392	100 µM	8.0 µl for 1ml	GTG AGG TAC CGG CCG TGG CG
Expected Bands:	Band Size: 247.0	Description: mTUBB5-247	(text displayed in the image)
	Band Size: 600.0	Description: 600 mut	(text displayed in the image)
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