PCR: sN1-Mut217
Name of Positive CTRL:sN1-Mut217
Annealing Temperature:60.0 °C
Number of cycles:35.0
Extension Time:50 s
Hot Start:Not yet
Primers:Primer:ID:Working Stock Concentration:Volume:Sequence (5'-3'):
m28s rDNA 225 F39010 µM10.0 µl for 1mlCAG ACG TGG CGA CCC GCT GA
m28s rDNA-225 R27610 µM10.0 µl for 1mlGCC TCA CAC CGT CCA CGG GC
pE22100 µM10.0 µl for 1mlcaaccgagctgaagcattctgcct
sN1-Mut217 rev495100 µM10.0 µl for 1mlaggcctccctcacacatgcttgag
Expected Bands:Band Size: 225.0Description: m28s 225(text displayed in the image)
Band Size: 425.0Description: 425 sN1(text displayed in the image)
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