PCR: | IL-2 |
Name of Positive CTRL: | IL-2 |
Annealing Temperature: | 64.0 °C | Number of cycles: | 35.0 |
Extension Time: | 45 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| oIMR0041 | 486 | 100 µM | 10.0 µl for 1ml | TCG AAT TCG CCA ATG ACA AGA CGC T |
| oIMR7338 | 487 | 100 µM | 20.0 µl for 1ml | CTA GGC CAC AGA ATT GAA AGA TCT |
| oIMR7339 | 488 | 100 µM | 20.0 µl for 1ml | GTA GGT GGA AAT TCT AGC ATC ATC C |
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Expected Bands: | Band Size: 324.0 | Description: 324 wt | (text displayed in the image) |
| Band Size: 500.0 | Description: 500 mutant | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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