PCR:	CD25
Name of Positive CTRL:	CD25
Annealing Temperature:	64.0 °C
Number of cycles:	12.0
Extension Time:	30 s
Hot Start:	Not yet
Primers:	Primer:	ID:	Working Stock Concentration:	Volume:	Sequence (5'-3'):
	IMR6916	491	100 µM	20.0 µl for 1ml	CTT GGG TGG AGA GGC TAT TC
	IMR6917	492	100 µM	20.0 µl for 1ml	AGG TGA GAT GAC AGG AGA TC
	oIMR0461mut	489	100 µM	10.0 µl for 1ml	CTG TGT CTG TAT GAC CCA CC
	oIMR0462 mut	490	100 µM	10.0 µl for 1ml	CAG GAG TTT CCT AAG CAA CG
Expected Bands:	Band Size: 146.0	Description: 146 wt	(text displayed in the image)
	Band Size: 280.0	Description: 280 mutant	(text displayed in the image)
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