PCR: | LysMCre wt |
Name of Positive CTRL: | LysMCre wt |
Annealing Temperature: | 62.0 °C | Number of cycles: | 35.0 |
Extension Time: | 60 s | Hot Start: | Not yet |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| MLys1 | 451 | 100 µM | 20.0 µl for 1ml | CTT GGG CTG CCA GAA TTT CTC |
| MLys2 | 452 | 100 µM | 20.0 µl for 1ml | TTA CAG TCG GCC AGG CTG AC |
| mTUBB5-247 F | 277 | 100 µM | 10.0 µl for 1ml | CGG CCA CCA TGA GCG GCG TC |
| mTUBB5-247 R | 392 | 100 µM | 10.0 µl for 1ml | GTG AGG TAC CGG CCG TGG CG |
|
Expected Bands: | Band Size: 247.0 | Description: mTUBB5-247 | (text displayed in the image) |
| Band Size: 350.0 | Description: wt | (text displayed in the image) |
|
|
More Information in PDF: | | view PDF | |
|