PCR: | LysMCre mut |
Name of Positive CTRL: | LysMCre mut |
Annealing Temperature: | 62.0 °C | Number of cycles: | 35.0 |
Extension Time: | 60 s | Hot Start: | HS 60 |
Primers: | Primer: | ID: | Working Stock Concentration: | Volume: | Sequence (5'-3'): |
| Cre8 | 453 | 100 µM | 20.0 µl for 1ml | CCC AGA AAT GCC AGA TTA CG |
| MLys1 | 451 | 100 µM | 20.0 µl for 1ml | CTT GGG CTG CCA GAA TTT CTC |
| mTUBB short F | 527 | 10 µM | 10.0 µl for 1ml | ACCGGGCCCTCACTGTGCCTG |
| mTUBB short R | 528 | 10 µM | 10.0 µl for 1ml | CGTCCACGGAAGACGGCGGCA |
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Expected Bands: | Band Size: 118.0 | Description: 118 mTUBBs | (text displayed in the image) |
| Band Size: 700.0 | Description: 700 mut | (text displayed in the image) |
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More Information in PDF: | | view PDF | |
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